Relative expression of IL-1β, TNFα, and RAD54B in LCLs from FA-A patients transfected with a Pre-miR control and Pre-miR-181c. In all cases, mRNA expression levels were analyzed by qPCR 2 days after transfection with a Pre-miR control (white bars) or Pre-miR-181c (black bars). Expression levels were related to the expression of the human β-actin gene and normalized to mean values corresponding to LCLs transfected with the Pre-miR control. Bars corresponding to IL-1β and TNFα expression represent mean values ± SEM of data deduced from the analysis of 3 FA patients. In the case of RAD54B, data from 2 patients are shown. For each determination, 3 independent experiments were conducted.