Comparative analysis of miRNA expression levels in primary blood cells from FA-A patients and HDs. The figure shows the relative expression of hsa-miR-99a, hsa-miR-133a, hsa-miR-135b, hsa-miR-139, hsa-miR-181c, hsa-miR-182, and hsa-miR-183 determined by qPCR in fresh MNCs obtained from HDs (■) and FA-A patients (▴). Top panels show miRNAs in which expression was not modified in samples from FA patients compared with those from HDs. Bottom panels show miRNAs significantly down-regulated in FA-A patients. Expression levels were related to the expression of the RNU6B gene and normalized to mean values corresponding to HD samples.