CLEC9A triggering does not affect DC phenotype or cytokine profile. Freshly isolated BDCA3⁺ mDCs were incubated overnight with mouse polyclonal anti–human CLEC9A or mouse IgG either in the presence or absence (control) of 2 μg/mL poly I:C and 4 μg/mL R848. (A) Expression of CD80 (black bars) and CD86 (white bars) was analyzed by flow cytometry after overnight incubation. Data are shown as mean fluorescence intensity of cells incubated with anti-CLEC9A relative to that of cells incubated with mouse IgG (MFI αCLEC9A/MFI IgG). (B) Cytokine and chemokine production after overnight incubation of BDCA3⁺ mDCs with anti–human CLEC9A or mouse IgG in the presence of 2 μg/mL poly I:C and 4 μg/mL R848. Data are shown as relative cytokine concentration (αCLEC9A/IgG). Graphs show the mean ± SEM of 3 independent experiments performed with BDCA3⁺ mDCs isolated from different donors. *P < .05.