BDCA3⁺ mDCs lose CLEC9A expression on maturation, whereas DEC205 is still expressed. Expression of CLEC9A (left) and DEC205 (right) on freshly isolated BDCA3⁺ mDCs (top; immature) and BDCA3⁺ mDCs stimulated overnight with poly I:C and R848 (bottom; pl:C + R848). Flow cytometry plots are representative examples of 4 independent experiments performed with DCs from different donors. Filled gray histograms represent isotype controls, and open histograms represent cells stained with specific antibodies.