Ang1 increases EC differentiation from mouse iPSCs and human ESCs. (A-C) iPSC-derived Flk-1⁺ MPCs were purified on E4.5 and cultured on OP9 cells with control buffer (Control) or Ang1. Analyses were performed on day 3. (A) Images showing CD144⁺ EC colonies. Nuclei were stained with Hoechst. Scale bar represents 200 μm. (B) Representative FACS analyses of the population of CD31⁺/CD144⁺ ECs. Numbers indicate percentages of ECs from Flk-1⁺ MPCs during differentiation. (C) Comparison percentages of CD31⁺/CD144⁺ ECs from purified Flk-1⁺ MPCs (n = 3). *P < .05 compared with control. (D-E) hESC-derived CD34⁺ cells were purified and cultured with Control or Ang1 and analyses were performed 9 days later. (D) Images showing CD144⁺ EC colonies. Nuclei were stained with Hoechst. Scale bars represent 200 μm. (E) Representative FACS analyses of the population of CD31⁺/CD105⁺ ECs. Numbers indicate percentages of ECs from CD34⁺ cells during differentiation. (F) Comparison percentages of CD31⁺/CD105⁺ ECs from purified CD34⁺ cells (n = 3). *P < .05 compared with control.