PGE2 inhibits DC-derived CXCL10, CCL5, and CCL19 secretion. Monocyte-derived DCs were matured with IFN-γ/FMKp in the presence of different concentrations of PGE2. After 6-hour maturation, DCs were washed to remove the stimulation medium and matured for an additional 18 hours in AIM V medium only. (A) Quantitative comparison of CXCL10, CCL5, and CCL19 production as measured by ELISA. Results shown are the mean + SEM of combined data of at least 5 different donors. (B) Percentage of NK cells migrated in 1.5 hours toward cell-free supernatant of IFN-γ/FMKp DCs matured in the presence of different concentrations of PGE2. Data were calculated as percentage of migrated NK cells. Results presented are the mean + SEM of combined data of 11 different donors. (C) Percentage of NK cells migrated in 1.5 hours toward cell-free supernatant of IFN-γ/FMKp DCs matured in the presence of PGE2 and supplemented with 50 ng/mL CCL5. Data were calculated as percentage of migrated NK cells, normalized to NK-cell migration toward IFN-γ/FMKp DCs matured without PGE2. Results presented are the mean + SEM of combined data of 4 different donors. (D) Quantitative comparison of CXCL10, CCL5, and CCL19 production after stimulation with different EP receptor agonists and cAMP as measured by ELISA. Results shown are the mean + SEM of combined data of at least 4 different donors. (E) Quantitative comparison of CXCL10, CCL5, and CCL19 production after stimulation with PGE2 either during or after DC maturation as measured by ELISA. Results shown are the mean + SEM of combined data of at least 4 different donors. *P < .05; **P < .01; ***P < .001.