American Society of Hematology

Figure 7

$Figure 7. IgG fractions and PBMCs of APS patients. Human pDCs were stimulated with IgG fractions of 4 patients with primary APS, 10 patients with secondary APS and SLE, and 10 healthy control donors. (A) Real-time RT-PCR after stimulation for 15 minutes to 6 hours. TLR7 mRNA expression was normalized to β-actin. Values represent mean ± SD of 5 independent experiments with all 24 IgG fractions *P < .05; **P < .005 for APS or SLE + APS versus SLE. (B) TNFα secretion was measured by the use of ELISA. Inhibition of TLR7 signaling was performed using IRS661 as described previously. *P < .005. (C) Production of ROS was determined by the use of flow cytometry as described in Figure 7E. *P < .005, **P < .02. (D) TLR7 mRNA levels in PBMCs from 8 different APS patients and 8 healthy controls were measured as duplicates by real-time RT-PCR. TLR7 mRNA was normalized to the expression of β-actin. *P < .005.$

IgG fractions and PBMCs of APS patients. Human pDCs were stimulated with IgG fractions of 4 patients with primary APS, 10 patients with secondary APS and SLE, and 10 healthy control donors. (A) Real-time RT-PCR after stimulation for 15 minutes to 6 hours. TLR7 mRNA expression was normalized to β-actin. Values represent mean ± SD of 5 independent experiments with all 24 IgG fractions *P < .05; **P < .005 for APS or SLE + APS versus SLE. (B) TNFα secretion was measured by the use of ELISA. Inhibition of TLR7 signaling was performed using IRS661 as described previously. *P < .005. (C) Production of ROS was determined by the use of flow cytometry as described in Figure 7E. *P < .005, **P < .02. (D) TLR7 mRNA levels in PBMCs from 8 different APS patients and 8 healthy controls were measured as duplicates by real-time RT-PCR. TLR7 mRNA was normalized to the expression of β-actin. *P < .005.

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