aPLs induce translocation of TLR8. Confocal images of MonoMac1 cells stained with anti-TLR8 (red) and anti-calnexin or anti-EEA1 (green). Nuclei were visualized with DAPI (blue). Before fixation cells were either stimulated with IgG or HL5B (100 ng/mL) for 30 minutes. In cells incubated with IgG (A) TLR8 colocalized with calnexin but not with EEA1. In addition, some TLR8 did not colocalize with either marker. In cells incubated with HL5B (B) colocalization of TLR8 with calnexin was not detectable, whereas there was significant overlap with EEA1. Again, some TLR8 did not colocalize with either marker. To allow better appreciation of the cellular features, a montage of representative cells from the same experiment on the same slide was prepared.