Conditional disruption of mouse Gabpa. (A) Schematic representation of relevant regions of the wild-type (+), floxed (loxP-flanked, or fl), and the recombined (deleted, or del) mouse Gabpa genomic loci. Rectangles indicate exons and numbered arrows refer to primers used to amplify the floxed (primers 1 and 2) or the deleted (primers 1 and 3) alleles. (B) Breeding schema used to generate mice that undergo conditional Gabpa deletion in response to pIC induction of Cre recombinase from the Mx1 Cre locus. Pairs of triangles indicate loxP sites of the floxed allele, single triangles indicate the deleted Gabpa locus; and rectangle indicates Cre recombinase. (C) PCR of genomic DNA from tail (Ta), liver (Li), bone marrow (BM), spleen (Sp), and thymus (Th) of control and KO mice for the fl and del Gabpa alleles and Cre recombinase. (D) Immunoblot of muscle (Mu), BM, S, and Th for Gabpα and β-actin.