Figure 5
CD81 colocalizes with F-actin and CD29 at the leading edge of migration-relevant membrane protrusions. Immunofluorescence staining of CD81 (red fluorescence, A) and detection of F-Actin localization by the use of Alexa488-phalloidin (green fluorescence, A). Immunofluorescence staining of CD81 (red fluorescence, B) and beta1 integrins (CD29, green fluorescence, B) in LPS-stimulated Mo-DC. The cells were adhered to two-dimensional fibronectin-coated surfaces (5μg/cm2). The fluorescence stainings are confocal images, focused on the basal plasma membrane of the cell. Bars represent 10 μm and 2 μm (magnified detail), respectively.
