Validation of PU.1–up-regulated miRs. (A) UCSC browser images of ChIPseq data from PUER cells after OHT treatment for the indicated time points and of primary macrophages³⁷  in alignment with global DNase HSS data of primary macrophages²²  (second rows from the bottom) and with sequence conservation tracks (bottom rows) according to the phylop algorithm (phast package, http://compgen.bscb.cornell.edu/phast/). Genomic coordinates are indicated at the top and miR positions, host genes and PU.1 occupancy peaks are shown at the bottom. (B) Kinetics of PU.1 induction of target miR expression in PUER cells compared with PU.1 KO controls. PUER and PU.1 KO cells were incubated with OHT (100nM) for the indicated time points. MiR expression was determined from total RNA by qRT-PCR compared with U6 RNA. PUER cells were induced with OHT (100nM) in the presence of cycloheximide (2 μg/mL; CHX) for 18 hours to inhibit protein translation. (C) MiR expression was determined from total RNA by qRT-PCR compared with U6 RNA. Error bars indicate SD.