Hematopoiesis and hematopoietic chimerism. (A) Hematopoietic chimerism, determined by flow cytometric analysis for GFP expression, of peripheral blood (top left; n = 10 for each cytokine group) and leukocyte subsets (bottom left; n = 10) at 1 week after cytokine treatment (13 weeks after BMT). Absolute cell counts of leukocytes (top middle; n = 10), neutrophils (top right; n = 10), and monocytes (bottom middle; n = 10) and donor chimerism of total bone marrow cells (bottom right; n = 5 for each cytokine group), all at 1 week after cytokine treatment. (B) The same analyses as in panel A with data obtained at 4 weeks after cytokine treatment (16 weeks after BMT; n = 5 for each cytokine group). All data are presented as mean ± SE. Statistically significant relationships are indicated. RBC indicates red blood cells; PLT, platelets; WBC, white blood cells; and BMC, bone marrow cells. CD11b, Gr-1, CD3, and B220 are lineage markers for monocytes, neutrophils, T lymphocytes, and B lymphocytes, respectively.