Fg (Fgα, Fgβ, and Fgγ chains) is significantly up-regulated in kidney cortex and medulla of male Wistar rats after 20 minutes of bilateral renal I/R injury compared with sham surgery. (A) A heatmap shows the expression levels of the most variable 1571 genes that were selected from 22 523 transcripts (see “Whole genome expression profiling and hierarchical clustering”). Coexpressed genes are grouped by hierarchical clustering. The samples are presented in order of time after ischemic injury (0, 6, 24, and 120 hours; triplicate for each). (B) Real-time PCR analysis in kidney. (C) Liver tissues for Fgα, Fgβ, and Fgγ chains, normalized using a housekeeping gene (Gapdh) and fold change determined over sham group (n = 5/group). *P < .05 as determined by 1-way ANOVA in comparison with sham rats.