![Figure 3. GATA-3 participates in HSC cell-cycle control. (A) Profile of annexin V and DAPI staining in BM LSK CD150+CD34− cells 3 weeks after poly(I:C) treatment. Numbers adjacent to the boxed areas indicate the mean percentage of cells in the gate. (B) Ratio of annexin V+DAPI− and annexin V+DAPI+ cells in LSK CD150+CD34− cells from adult Gata3flox/flox [f/f(−)], Gata3flox/+:TgMx1Cre [f/+(+)], and Gata3flox/flox:TgMx1Cre [f/f(+)] mice 3 weeks after poly(I:C) treatment. Data represent the summary of at least 5 mice of each genotype from 3 independent experiments with SEM. NS indicates not significant. (C) Profile examining the number of cells in cycle assessed after 19 hours of BrdU labeling with DNA content (DAPI) in BM LSK CD150+CD48− (top panel) and LSK CD150+CD48−CD34− (bottom panel) populations 3 weeks after poly(I:C) treatment. Numbers in the boxed areas indicate the mean percentage of cells in each gate. (D) Ratio of BrdU+ cells (S phase) in BM LSK (left group), LSK CD150+CD48− (middle group), and LSK CD150+CD48−CD34− (right group) populations 3 weeks after poly(I:C) treatment. Data represent the summary of 5 mice of each genotype from 5 independent experiments with SEM. *P < .05; #P < .002. NS indicates not significant. (E) Cell-cycle analysis using intracellular Ki67 and DNA content (DAPI) staining in BM LSK CD150+CD34− cells 3 weeks after poly(I:C) treatment. Numbers adjacent to the boxed areas indicate the mean percentage of cells in gate. (F) Ratio of cells in the G0, G1, and S + G2/M phases in BM LSK CD150+CD34− cells from adult f/f(−) (black bars), f/+(+) (gray bars), and f/f(+) (white bars) 3 weeks after poly(I:C) treatment. Data represent the summary of at least 5 mice of each genotype from 3 independent experiments with SEM. *P < .008; #P < .02; +P < .03. NS indicates not significant. (G) Profile of DNA content (DAPI) staining in BM LSK cells without (top panels) or with (bottom panels) 5-FU treatment for 6 days; cells were analyzed 3 weeks after poly(I:C) treatment. Numbers above the indicator bars quantify the mean percentage of cells in that gate. (H) Ratio of cells in the S + G2/M phase in the LSK population (top) and the absolute LSK cell numbers (bottom) per mouse (2 femurs plus 2 tibias) either without (−) or with (+) 5-FU treatment. Data represent the summary of at least 6 mice of each genotype and treatment groups from 3 independent experiments with SEM. *P < .001; #P = .05; +P < .03. NS indicates not significant.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/119/10/10.1182_blood-2011-07-366070/4/zh89991287420003.jpeg?Expires=1723440376&Signature=jwHKzEYM5vsxmkZCU-UHgjSZUn2G2yjj5pOzKYGHeibWtvMcLU953GsH5yL1wooLfBCK6gTrpKsJvWazIM1aCsLPXfTDGHUUSBRQsKrw37KBut3tNpgyK1aB-HKZyREofKTxDWvF1jh-bzKXoyh9byTLoEJ3RDzO~sSnogEan5Yku07WZggKYcKubjVvqhQSYY4KEhC11oQtMdKfbVogqHZy94DeWhVWf1fXK6kBZjxvil0qMcjavcFzS~JC5SgupAtBL5OX8a90hhFGTMNwX7OnMHtRWBsWoRIcbnkQ4Vhqr921pgMxCOcTa4cU5kpBlqLUIq8q1fX6yqc31020hg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
GATA-3 participates in HSC cell-cycle control. (A) Profile of annexin V and DAPI staining in BM LSK CD150+CD34− cells 3 weeks after poly(I:C) treatment. Numbers adjacent to the boxed areas indicate the mean percentage of cells in the gate. (B) Ratio of annexin V+DAPI− and annexin V+DAPI+ cells in LSK CD150+CD34− cells from adult Gata3flox/flox [f/f(−)], Gata3flox/+:TgMx1Cre [f/+(+)], and Gata3flox/flox:TgMx1Cre [f/f(+)] mice 3 weeks after poly(I:C) treatment. Data represent the summary of at least 5 mice of each genotype from 3 independent experiments with SEM. NS indicates not significant. (C) Profile examining the number of cells in cycle assessed after 19 hours of BrdU labeling with DNA content (DAPI) in BM LSK CD150+CD48− (top panel) and LSK CD150+CD48−CD34− (bottom panel) populations 3 weeks after poly(I:C) treatment. Numbers in the boxed areas indicate the mean percentage of cells in each gate. (D) Ratio of BrdU+ cells (S phase) in BM LSK (left group), LSK CD150+CD48− (middle group), and LSK CD150+CD48−CD34− (right group) populations 3 weeks after poly(I:C) treatment. Data represent the summary of 5 mice of each genotype from 5 independent experiments with SEM. *P < .05; #P < .002. NS indicates not significant. (E) Cell-cycle analysis using intracellular Ki67 and DNA content (DAPI) staining in BM LSK CD150+CD34− cells 3 weeks after poly(I:C) treatment. Numbers adjacent to the boxed areas indicate the mean percentage of cells in gate. (F) Ratio of cells in the G0, G1, and S + G2/M phases in BM LSK CD150+CD34− cells from adult f/f(−) (black bars), f/+(+) (gray bars), and f/f(+) (white bars) 3 weeks after poly(I:C) treatment. Data represent the summary of at least 5 mice of each genotype from 3 independent experiments with SEM. *P < .008; #P < .02; +P < .03. NS indicates not significant. (G) Profile of DNA content (DAPI) staining in BM LSK cells without (top panels) or with (bottom panels) 5-FU treatment for 6 days; cells were analyzed 3 weeks after poly(I:C) treatment. Numbers above the indicator bars quantify the mean percentage of cells in that gate. (H) Ratio of cells in the S + G2/M phase in the LSK population (top) and the absolute LSK cell numbers (bottom) per mouse (2 femurs plus 2 tibias) either without (−) or with (+) 5-FU treatment. Data represent the summary of at least 6 mice of each genotype and treatment groups from 3 independent experiments with SEM. *P < .001; #P = .05; +P < .03. NS indicates not significant.
