SPR analysis of the p17/CXCR1 and p17/CXCR2 interaction. (A) P17 (straight lines) or SDF-1α (dashed lines, both at 1.25μM) were injected over BIAcore CM5 sensor chips coated with a mAb to GST (blue lines) or in the presence of CXCR1 recombinant protein with GST tag (black lines). (B) Overlay of blank-subtracted sensorgrams resulting from the injection of increasing concentrations of p17 over the GST-CXCR1 surface. Black lines represent the experimental data. Red lines represent the fits.