Identification of 4 cDC subsets in human SLN. (A) Flow cytometric dot plots for CD11c, CD1a, and CD14: gating of 4 different cDC subsets in human SLN, ie, 2 CD1a⁺ subsets, CD11c^hi CD1a^int and CD11c^int CD1a^hi (top) and 2 CD1a⁻ subsets, CD1a⁻ CD11c⁺ CD14⁻ and CD1a⁺ CD11c⁻ CD14⁺ (gated to exclude CD1a⁺ cells). (B) Frequencies of the 4 cDC subsets within SLN single-cell suspensions (n = 14). (C) Forward scatter (FSC) and side scatter (SSC) plots for the 4 cDC subsets, gated on CD11c^hi CD1a^int, CD11c^int CD1a^hi, CD1a⁻ CD11c⁺ CD14⁻, and CD1a⁻ CD11c⁺ CD14⁺ and back-gated onto forward/side scatter. (D) Morphologic characterization (cytochemical May-Grünwald-Giemsa staining) of sorted CD11c^hiCD1a^int, CD11c^intCD1a^hi, CD1a⁻CD11c⁺CD14⁻, and CD1a⁻CD11c⁺CD14⁺ cells, isolated (FACS-sorted) from human SLN. (E) Immunocytochemical staining for CD1a, CD14, and langerin cells on SLN imprints, arrows indicate CD14⁺ cell protrusions (magnification 400×).