The gene expression profile in Y747A-mutant HSCs differed from that in WT and integrin-β3^−/− HSCs. (A) After whole transcriptome analysis of WT, integrin-β3^−/− and Y747A HSCs using SOLiD sequencing, hierarchical cluster analysis was performed after filtration based on ANOVA (P < .05) and a > 2-fold change against WT (at least one pair). Up-regulated and down-regulated genes are shown in red and blue, respectively. (B) GSEA was performed using the whole transcriptome of WT and Y747A HSCs. The pie chart represents the distribution of 102 gene sets up-regulated in WT HSCs, compared with Y747A HSCs, into the indicated categories. The threshold was set at P < .05 and FDR (q < 0.25).