Monocytes cocultured with B-cell lymphoma cells show CXCL10 expression and protumoral effects. (A) CXCL10 expression by monocytes cocultured with or without DAUDI cells for 18 hours. Thereafter, the transwells containing DAUDI cells were removed; and the monolayers werre washed, incubated in fresh media for 1 hour, and stimulated with or without LPA (100 ng/mL) for 4 hours. Mo indicates monocytes alone; and Mo + DAUDI, monocytes cocultured with DAUDI cells. *P < .05, versus Mo. **P < .02, versus Mo + LPA. Migration (B) and invasion (C) of DAUDI cells in response to supernatants from DAUDI-cocultured monocytes, in the presence or absence of anti-CXCL10 (10μg/mL; left panels). *P < .002, versus Mo + DAUDI (with or without LPA). Right panels: Migration and invasion of DAUDI cells in response to recombinant human CXCL10. *P < .002, versus media. Data are mean ± SD (A) and mean ± SEM (B-C) of a representative experiment. (D) MMP9 activity by zymography. (E) MMP9 gene expression was assessed in DAUDI cells in response to recombinant human CXCL10. Data are mean ± SEM (n = 3). *P < .001, versus media.