Up-regulated CXCL10 expression in monocytes isolated from BCP-ALL patients. (A) Representative flow cytometric analysis of the BM cALL blasts. cALL blasts are indicated by the CD19⁺/CD14⁻ cells, whereas tumor-associated monocytes (ALL-Mo) were indicated by the CD19⁻/CD14⁺ cells. Numbers in parentheses indicate relative percentage of cALL cells and ALL-Mo. (B) Monocytes (ALL-Mo) as well as CD19⁺ cALL cells (cALL) were isolated from the blasts by FACS sorting and checked for the expression of CXCL10 by quantitative PCR. Monocytes from normal donors (Mo) were used as a control. (C) CXCL10 levels in the plasma of patients (n = 13) and healthy donors (n = 6) determined by Bioplex assay. Data are mean ± SEM. *P < .03, versus healthy. (D) Expression of a panel of inflammatory and protumoral genes in ALL-Mo by quantitative PCR. Data represent fold change expression of genes with respect to their basal line expression in monocytes from healthy donors. (E) Expression of a panel of inflammatory and protumoral genes in monocytes (ALL-Mo) isolated from the peripheral blood of cALL patients by quantitative PCR. (F) Quantitative PCR analysis of the indicated genes in ALL-Mo isolated from pre-B ALL patient blasts, compared with monocytes from healthy donors. Data are mean ± SEM (n = 2). (B,D-E) Data are mean ± SEM (n = 4). *P < .05, versus Mo.