Accuracy of the optimized quantitative PCR assay in a phase 2 study of T + E study in elderly AML. (A) ROC analysis using complete remission (CR) only as response. AUC indicates area under the ROC curve. (B) A 2 × 2 contingency table using the optimal cutoff and 2-gene assay performance characteristics. NR indicates no response; PPV, positive predictive value; NPV, negative predictive value; ORR, overall response rate; Spec, specificity; and Sens, sensitivity. (C) Kaplan-Meier analysis of patients stratified using an optimal ratio cut-off of 5.2. HR indicates hazard ratio.