Figure 2
Biologic activity of C2-2b-2b. (A-B) Binding of mAbs and mAb-IFNα to live NHL cells. After binding of the indicated constructs, cells were probed with PE-conjugated goat anti–human Fc and analyzed by flow cytometry. MFI indicates mean fluorescence intensity; n = 5000 cells; error bars, 95% confidence interval. (A) Raji (black bars) or RL (hatched bars) cells were incubated at 4°C for 1 hour in the presence of 5nM of the indicated construct. (B) RL cells were incubated at 4°C for 1 hour in the presence of 0.2-50nM of hL243 IgG1, C2-2b-2b, or 20-2b-2b. (C) IFNα2-specific activities were determined with a cell-based reporter gene assay.
