Effect of platelet transfusion on Western blot analysis. (A) Western blotting analysis for Munc13-4 expression using platelets and PBMCs from an FHL3 patient (P5) receiving platelet transfusions during the acute phase of the disease. (B) The expression of Munc13-4 was compared between PBMCs obtained by density gradient centrifugation (DGC) and CD45⁺ cells obtained by magnetic sorting from healthy controls. A representative result of 3 independent experiments is shown.