Regulation of lymphatic differentiation by the Notch pathway. (A) qPCR analysis for LYVE1 and podoplanin expression in lymphatic endothelial cells treated with 100nM DBZ or transfected with siRNA targeting Dll4 or Notch1 (n = 3; *P < .05). (B) LYVE1 (green) and podoplanin (red) staining of P7 tail dermis isolated from neonates treated with PBS (control), αNotch1, αDll4, ALK1-Fc, and VEGFR3-Fc (10 mg/kg on P2/5). Scale bar represents 200 μm.