American Society of Hematology

Figure 4

$Figure 4. Bone marrow failure in Mpl−/−Erg+/Mld2 mice. (A) Survival of Mpl−/−Erg+/+ (broken line, n = 172) and Mpl−/−Erg+/Mld2 (solid line, n = 58) mice. P < .0001 by log-rank analysis, with median survival of 115 days for Mpl−/−Erg+/Mld2 mice. (B) Representative flow cytometric analysis of the LSK fraction from Mpl+/+Erg+/+ (n = 6), Mpl−/−Erg+/+ (n = 6), and Mpl−/−Erg+/Mld2 (n = 6) mice at 7-10 weeks of age. (Left panels) The number (mean ± SD) of Lin− cells as a percentage of viable bone marrow cells. (Right panels) The number of Kit+ Sca-1+ cells as a percentage of Lin− cells. (C) Survival of mice transplanted with of 3 × 106 unfractionated bone marrow cells from Mpl−/−Erg+/+ (○) and Mpl−/−Erg+/Mld2 (●, 2 independent donors) mice, with 5 recipients per donor.$

Bone marrow failure in Mpl^−/−Erg^+/Mld2 mice. (A) Survival of Mpl^−/−Erg^+/+ (broken line, n = 172) and Mpl^−/−Erg^+/Mld2 (solid line, n = 58) mice. P < .0001 by log-rank analysis, with median survival of 115 days for Mpl^−/−Erg^+/Mld2 mice. (B) Representative flow cytometric analysis of the LSK fraction from Mpl^+/+Erg^+/+ (n = 6), Mpl^−/−Erg^+/+ (n = 6), and Mpl^−/−Erg^+/Mld2 (n = 6) mice at 7-10 weeks of age. (Left panels) The number (mean ± SD) of Lin⁻ cells as a percentage of viable bone marrow cells. (Right panels) The number of Kit⁺ Sca-1⁺ cells as a percentage of Lin⁻ cells. (C) Survival of mice transplanted with of 3 × 10⁶ unfractionated bone marrow cells from Mpl^−/−Erg^+/+ (○) and Mpl^−/−Erg^+/Mld2 (●, 2 independent donors) mice, with 5 recipients per donor.

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