American Society of Hematology

Figure 1

$Figure 1. Steady-state HSC activity in Erg+/Mld2 mice. (A) Representative flow cytometric analyses of the LSK bone marrow fraction with CD150 and CD48 SLAM markers. The box indicates the CD150+CD48−, HSC-enriched population as a percentage of viable bone marrow cells at 8 weeks of age. Data are mean ± SD of 6 mice per genotype. (B) Bone marrow cellularity represented as number of viable nucleated cells per femur, with (C) numbers of total LSK cells per femur at 8 weeks and 12 months of age and (D) CD150+CD48− LSK cells at 8 weeks and 6 months of age expressed as the number of cells per femur (left panel) and percentage of viable nucleated bone marrow cells (right panel). Data are mean ± SD of 6 mice per genotype for each time point shown. *P < .02. (E) Relative contribution to LSK cells, hematopoietic progenitor (Lin−Sca−cKit+), myeloid, T, and B cells in recipients of mixtures of 12-month-old and 10-week-old genotype-matched bone marrow in a 1:1 competitive transplant. Data are mean ± SD for each reconstituted fraction for 3 independent competitive transplant experiments. P > .05 for comparison of data from Erg+/Mld2 and wild-type donors for each population examined.$

Steady-state HSC activity in Erg^+/Mld2 mice. (A) Representative flow cytometric analyses of the LSK bone marrow fraction with CD150 and CD48 SLAM markers. The box indicates the CD150⁺CD48⁻, HSC-enriched population as a percentage of viable bone marrow cells at 8 weeks of age. Data are mean ± SD of 6 mice per genotype. (B) Bone marrow cellularity represented as number of viable nucleated cells per femur, with (C) numbers of total LSK cells per femur at 8 weeks and 12 months of age and (D) CD150⁺CD48⁻ LSK cells at 8 weeks and 6 months of age expressed as the number of cells per femur (left panel) and percentage of viable nucleated bone marrow cells (right panel). Data are mean ± SD of 6 mice per genotype for each time point shown. *P < .02. (E) Relative contribution to LSK cells, hematopoietic progenitor (Lin⁻Sca⁻cKit⁺), myeloid, T, and B cells in recipients of mixtures of 12-month-old and 10-week-old genotype-matched bone marrow in a 1:1 competitive transplant. Data are mean ± SD for each reconstituted fraction for 3 independent competitive transplant experiments. P > .05 for comparison of data from Erg^+/Mld2 and wild-type donors for each population examined.

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