Figure 7
Figure 7. LMP1 and RON are coexpressed in EBER-positive B cells in 2 PTLD biopsies. Formalin-fixed, paraffin-embedded PTLD consecutive sections of case nos. 1 and 4 were stained for the expression of CD20, EBER, LMP1, and RON. Positive CD20 staining is seen as a dark brown precipitate on the surfaces of the B cells. The EBER signal was characterized by a dense purple. Positive immunoreactivity against LMP1 and RON was seen as a light brown color. Co-localization among EBER, CD20, LMP1, and RON was observed using a Zeiss photomicrographic system (Axioskop 40 FL) equipped with Image-Pro Plus software and indicated by arrow. Magnification ×400 (scale bar, 25 μm).

LMP1 and RON are coexpressed in EBER-positive B cells in 2 PTLD biopsies. Formalin-fixed, paraffin-embedded PTLD consecutive sections of case nos. 1 and 4 were stained for the expression of CD20, EBER, LMP1, and RON. Positive CD20 staining is seen as a dark brown precipitate on the surfaces of the B cells. The EBER signal was characterized by a dense purple. Positive immunoreactivity against LMP1 and RON was seen as a light brown color. Co-localization among EBER, CD20, LMP1, and RON was observed using a Zeiss photomicrographic system (Axioskop 40 FL) equipped with Image-Pro Plus software and indicated by arrow. Magnification ×400 (scale bar, 25 μm).

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