Figure 4
The LMP1-NF-κB pathway is required for RON expression in EBV-harboring LCLs. (A) LCL-1 cells were treated with 2.5μM or 5μM BAY11-7082 for 48 hours. The RON transcripts were measured by RT-Q-PCR and the relative fold expression of RON was normalized to the amounts of RON transcripts in untreated LCLs (top panel). The expression of RON, p-IκB-α, LMP1, and GAPDH proteins was measured by Western blotting (bottom panel). (B) 2 × 105 LCL-1 cells were infected with control shRNA or LMP1 shRNA-expressing lentiviruses at an MOI of 4 for 5 days. A ChIP assay of RON promoter was carried out as described in Figure 3.
