Phosphorylation patterns of normal and ChAc RBC membranes by exogenous Lyn. Membranes of high-density fraction (F2) of RBCs from control (C) and ChAc subjects were incubated without (lanes 1 and 5) or with exogenous Lyn (lanes 2-4 and 6-8) and with GST-Lyn/SH3 (lanes 3 and 7) or GST-Lyn/SH2 (lanes 4 and 8), respectively. Samples were then analyzed by Western blot (Wb) analysis with anti–phosphotyrosine antibodies. Similar data were obtained with F1 control and ChAc RBCs (data not shown). The membranes were reprobed with anti–actin antibody as loading control.