Tcl1 and Atm affect the NF-κB pathway in CLL patient samples and Tcl1 transgenic mice. (A) B-CLL samples were transfected with si-TCL1 for 36 hours or treated with Kudos (10μM, 12 hours) or both and in all cases treated with hydroxyurea, 50mM 3 hours. IκBα and Egr1 expression was detected by Western blot. (B) B-cell CD19 isolated from mouse spleen was lysed and analyzed by Western blot with indicated antibodies. Spleens were excised from wild-type and Tcl1 transgenic mice at 3, 10, and 15 months of age. Data are representative of 3 independent experiments. (C) Whole B-CLL cell lysates were immunoprecipitated with anti-Tcl1 antibody. The immunoprecipitates were analyzed by immunoblotting (IB) with anti-Atm and anti-Tcl1 antibodies. Input: total lysate.