After HCT, donor cells migrate to sdf-1a:DsRed2 structures in recipients. (A) Photo composite of images taken with a 10×/0.3 NA objective of an ex vivo kidney of Tg(sdf-1a:DsRed2) 4 days after preconditioning with 30 Gy cesium radiation and 2 days after transplant with 100 000 Green Glofish MyeloMonocyte cells. For clarity, locations of donor-derived GFP⁺ cells are marked with a white dot (left panel). GFP⁺ donor-derived cells can be found adjacent to sdf-1a:DsRed2⁺ tubules. Photos taken with a 40×/0.6 NA objective (right panels). (B) Photo composite of a Tg(sdf-1a:DsRed2) fish 5 days after preconditioning with 25 Gy x-ray radiation and 3 days after transplantation with 1 × 10⁶ Green Glofish WKM cells. Photos taken with a Leica M165 FC fluorescent microscope, Planapo 1.6×/.05 NA objective, Leica DFC340 FX camera, and Leica Application Suite software (left), and detail of regions of concentrated localizations of sdf-1a:dsRed cells and GFP⁺ donor-derived cells. Photos taken with a 10×/0.3 NA objective and IP Lab V4.0.8 acquisition software (right).