sdf-1a expression reported by Tg(sdf-1a:DsRed2). (A) In vivo fluorescence signal reporting sdf-1a expression in the adult transgenic, revealing expression in the skin and gills (imaged with a Leica MZ FLIII fluorescence stereomicroscope). (B) In vivo sdf-1a:DsRed2 (red) indicates sdf-1a expression in chevron-shaped structures in the skin, and fli:EGFP (green) expression marks vessels in a double transgenic fish with both reporters. Bar represents 200 μm. (C) sdf-1a:DsRed2 expression in renal tubules that do not report expression of atp1a1a.4, in a fish transgenic for both reporters. Bar represents 50 μm. (D) sdf-1a:DsRed2 expression in distal renal tubules that also express atp1a1a.4, in a fish transgenic for both reporters. Bar represents 125 μm. (E) fli:EGFP expression marking vessels of a glomerulus, and sdf-1aDsRed2 expression in an immediately adjacent (proximal) renal tubule of a transgenic fish with both reporters, imaged in PBS. Bar represents 50 μm. (F) An isolated kidney tubule showing sdf-1a:DsRed2 signal in renal tubule cells. Green represents WGA-AF488 applied to fresh tubules at 10 μg/mL for 20 minutes on ice (a concentration calculated to produce widespread nonspecific labeling for purposes of revealing basic tubule morphology). After staining, specimen was rinsed in PBS and immediately imaged in glycerol compressed between 2 glass coverslips, using a 40× oil-immersion objective.