SDF-1 chemoattracts zebrafish WKM cells. (A) Two-hour in vitro transwell migration assay, with migration ratios calculated from hematocytometer total cell counts. Untreated, n = 4 experiments; and AMD3100-treated, n = 2 experiments. Error bars represent SEM. (B) Fluorescence-activated cell sorter analysis of migrated cells from in vitro migration assays. Percentage myelomonocytes are calculated from a cell population with FSC/SSC properties of lymphocytes, precursors, and myelomonocytes. Bars show the mean. Untreated, n = 4 experiments; and AMD3100-treated, n = 3 experiments. ***P < .001 (analysis of variance with Tukey postanalysis). **.001 < P < .01 (analysis of variance with Tukey postanalysis). *.01 < P < .05 (analysis of variance with Tukey postanalysis). (C) odysseus mutant WKM was labeled with vital dye and transplanted into AB and WT fish preconditioned with 20 Gy x-ray irradiation. Recipient kidneys were harvested and analyzed by flow cytometry 1 day after transplantation. Graphs represent the number of donor-derived cells detected per 60 000 total cells. Bars represent the mean; n = 2 experiments, unpaired t test.