In situ hybridization of zebrafish tissues. Whole-mount in situ hybridization was performed on tissues from adult zebrafish skin (A,E), gills (B,F), and kidney (C-D,G-H). (A) sdf-1a expression in a chevron-shaped pattern in the skin. Bar represents 150 μm. (E) sdf-1b expression in skin structures with vascular morphology. Bar represents 150 μm. (B) sdf-1a expression in gills. Bar represents 250 μm. (F) sdf-1b expression in gills. Bar represents 250 μm. (C) sdf-1a expression in the kidney. Arrows indicate kidney tubule. Bar represents 100 μm. (D) Arrow indicates sdf-1a expression in renal tubules cells. Bar represents 25 μm. (G) sdf-1b expression in the kidney, showing predominant signal located in areas of glomeruli and adjacent tubules. Bar represents 100 μm. (H) Arrows indicate sdf-1b expression in proximal renal tubule. Bar represents 25 μm.