RORγt is partly required for the intrathymic development of IL-17–producing γδ T cells. (A-C) IL-17–producing γδ T cells in RORc^+/+ (+/+), RORc^+/− (+/−), and RORc^−/− (−/−) mice. Single-cell suspensions of fetal (E16) and adult thymocytes (6 weeks old; A-B) or PEC, spleen, and LPL (4 weeks old; C) from RORc^+/+ (+/+), RORc^+/− (+/−), and RORc^−/− (−/−) mice were stimulated with PMA and ionomycin and analyzed for intracellular staining for IL-17. (A) Representative dot plots of intracellular staining for IL-17 in fetal (top panels) and adult (bottom panels) thymi are shown after gating on CD3⁺ cells. The number in the top right quadrant indicates the percentage of IL-17⁺ cells in γδTCR⁺ cells. (B-C) Absolute numbers of IL-17⁺ γδ T cells in each organ are shown. Data shown are the means ± SD of 4-5 mice. *P < .05; **P < .01; ***P < .001. N.S. indicates statistically not significant between groups. Data are representative of 3 independent experiments.