Figure 4
![Figure 4. Effect of silencing Bim, Noxa, and Puma on ABT-737–induced apoptosis. Knock-down efficiency was analyzed by Western blotting of each protein in untreated and 0.4μM ABT-737 treated samples (left panels). Activity of ABT-737 was analyzed by annexin/PI staining after knock-down of each protein (right panels). Data are mean ± SD of at least 3 independent experiments. P values: 8226, silencing of all 3 BH3 proteins are significantly different from [si(−)] control (P < .005), with the exception of siPuma at 0.8μM ABT-737 (not significant). KMS11, all values are significantly different from control (P < .005), with the exception of siNoxa at 1.6 and 3.2μM (not significant). MM.1s, siBim significantly different from control (P < .005).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/118/5/10.1182_blood-2011-01-327197/4/zh89991175570004.jpeg?Expires=1722785498&Signature=X0mOKTaC8m1NrIR9fhitda82RcEbVLQyaq4bN6DgpWJQxfNm1O-jSXSQTYxgi6LVuGvAY5otsH3GvhYhmatexUoV78KmMBEKps2Dcs0~LvwCZ10UF1acPHqNbUs~5m3Alza9v0EZD-X1-NwKHito5e5alhxo0Su5zK31aNHrx-MXAi-~2vIeTXYuLBvdKY8T2jtE6JveJi6-JnWqmdTOHcoB4HjDvbC5WypQV~hrtfCcqb3QVGyvIAIWQos~OHe6PbBTImFDB8zGoVAAfrNxrsBFRerVB6~9c8xPFU0YNe2RujmgnT1fo-OH5N4lY4fY-3vF1GsOs3u8IoBK8o5Ddw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of silencing Bim, Noxa, and Puma on ABT-737–induced apoptosis. Knock-down efficiency was analyzed by Western blotting of each protein in untreated and 0.4μM ABT-737 treated samples (left panels). Activity of ABT-737 was analyzed by annexin/PI staining after knock-down of each protein (right panels). Data are mean ± SD of at least 3 independent experiments. P values: 8226, silencing of all 3 BH3 proteins are significantly different from [si(−)] control (P < .005), with the exception of siPuma at 0.8μM ABT-737 (not significant). KMS11, all values are significantly different from control (P < .005), with the exception of siNoxa at 1.6 and 3.2μM (not significant). MM.1s, siBim significantly different from control (P < .005).
