Knockdown of metap2 perturbed angiogenesis but not vasculagenesis. (A) Confocal microscopy of Tg(fil1:gfp) at 48 hpf comparing the development of dorsal aorta (DA), dorsal vein (DV), and intersegmental vessel (ISV) in the trunk region between control and metap2^MO. (B) WISH comparing expression of efnb2a (control, 92.4% ± 2.3% with normal efnb2a expression in total 92 embryos; metap2^MO, 91.4% ± 2.7% with normal efnb2a expression in total 93 embryos; n = 3) and flt4 (control, 95.5% ± 1.4% with normal flt4 expression in total 88 embryos; metap2^MO: 95.3% ± 1.0% with normal flt4 expression in total 86 embryos; n = 3) at 30 hpf between control and metap2^MO. Black arrowheads denote efnb2a expression along DA, and the dashed line outlines the extension of the yolk. (C) Fluorescent microscopy of Tg(fil1:gfp) at 48 hpf comparing the development of DA, DV, and ISV in the trunk region between control and metap2^MO rescued by metap2 mRNA (control, 96.8% ± 1.4% with normal ISV patterning in total 93 embryos; metap2^MO, 80.3% ± 3.4% with perturbed ISV patterning in total 71 embryos; metap2^MO rescue, 72.5% ± 4.0% with normal ISV patterning in total 80 embryos; n = 3). Bars represent 50 μm.