Knockdown of metap2 affected embryonic hematoporesis. (A) WISH comparing expression of mpo at 18 hpf between control and metap2^MO (control, 90.6% ± 0.8% with normal mpo expression in total 85 embryos; metap2^MO, 72.1% ± 2.6% with increased mpo expression in total 68 embryos; n = 3). (B-C) WISH comparing expression of c-myb between control and metap2^MO at 36 hpf (B; control, 93.8% ± 1.3% in total 128 embryos with normal c-myb expression; metap2^MO, 82.6% ± 2.7% in total 121 embryos with reduced c-myb expression; n = 5) and 48 hpf (C; control, 89.2% ± 1.0% in total 148 embryos with normal c-myb expression; metap2^MO, 78.9% ± 3.0% in total 147 embryos with reduced c-myb expression; n = 5). (D) WISH showing the expression of c-myb at 36 (rescued c-myb expression, 75.3% ± 3.8% in total 72 embryos, n = 3) and 48 hpf (rescued c-myb expression, 77.0% ± 4.0% in total 87 embryos, n = 3) in metap2^MO rescued by metap2 mRNA. Bars represent 250 μm.