DMOG treatment causes a change in direction of neutrophil movement independent of neutrophil speed and meandering. (A) Photomicrographs of a 3-dpf photoconverted, DMSO-treated, lyz:Kaede embryo at 0 hpc. The white box indicates the area of photoconverted neutrophils. The bottom panel shows the regions to which neutrophils migrated in subsequent panels (D-F). (B) Photomicrographs of examples of DMSO (top panel)– and DMOG (bottom panel)–treated embryos with the tracks of neutrophils over the first 3.5 hpc shown. The dashed line indicates the outline of the embryo. (C) Tracking of neutrophils over the first 3.5 hpc in the resolution phase of inflammation showed no difference in the speed of neutrophil migration (top panel) or meandering index (displacement/path length; bottom panel) in DMOG-treated larvae compared with DMSO controls. (D) Leukocytes leave the photoconversion site at the same rate in DMOS- and DMOG-treated embryos over 3.5 hpc. (E) Number of photoconverted leukocytes moving away from the wound is shown over 3.5 hpc in DMSO- and DMOG-treated embryos. (F) More neutrophils remain and patrol the injury site in DMOG-treated embryos, because a greater number of leukocytes move within the injury region, ventrally away from the patch area. (D-F) Data shown are mean ± SEM, n = 14 performed as 3 independent experiments. Line of best fit shown is calculated by linear regression. P values shown are the differences between the 2 slopes.