DMOG retains neutrophils in the region of tissue injury. (A) Photomicrographs of a 3-dpf photoconverted, DMSO-treated, lyz:Kaede embryo at 0 hours postconversion (hpc). The white dashed line indicates the border of the site of transection area. The cells to the right of the line were photoconverted, leaving them with red fluorescence rather than green. The bottom fluorescent panel shows the area of interest where neutrophils migrated away from the injury site shown in a subsequent figure (D). (B) Photomicrographs of the same embryo as in panel A at 0 and 3.5 hpc. The red channel only is shown, as a binary image. Between 0 and 3.5 hpc, photoconverted cells have migrated away from the site of transection. (C) Photomicrographs of a DMOG-treated embryo at 0 and 3.5 hpc converted into black and white. Fewer photoconverted leukocytes have migrated away from the site of transection. (D) Plot showing the number of photoconverted leukocytes leaving the area of transection over 3.5 hpc in DMSO- and DMOG-treated embryos. Data shown are mean ± SEM, n = 9 performed as 2 independent experiments. Line of best fit shown is calculated by linear regression. P value shown is for the difference between the 2 slopes.