Apoptosis and cell-cycle analyses. (A) Apoptosis analysis of freshly isolated BM cells from age-matched 8- to 12-week-old mice. The graph shows the percentage of Annexin V⁺ cells in LSK cells from Msi2^+/+ (n = 5) and Msi2^Gt/Gt mice (n = 8). (B) Cell-cycle analysis of cells as above. FACS analysis of cells stained with an antibody that detects proliferating cells (Ki67) and DAPI to determine the relative DNA content on a per-cell basis. (C) Percentage of LSK cells in the G0, G1, and S-G2/M phases (n = 6 for both groups).