A retroviral screen for potential regulators of HSC function. (A) Experimental design of BM transplantation assays using donor cells infected with RV-GFP retrovirus. The radioactivity symbol denotes that the mice have been lethally irradiated. (B) Agarose gel showing the results of LM-PCR, with the arrowhead pointing to the internal control band (1 kb). M indicates marker DNA. (C) Schematic illustration of the Msi2 gene showing the provirus insertion site (not to scale) and the distance between the insertion and the transcription start site (TSS). LTR indicates long terminal repeat. (D) FACS plot from granulocyte-macrophage colonies obtained from a secondary recipient. (E) Msi2 expression as determined by qRT-PCR from sorted CD45.1⁺GFP⁻ and CD45.1⁺GFP⁺ donor populations.