MVD analysis before and after transplantation. Matrigel implants were harvested at multiple time points before and after transplantation. (A) Diagram of transplantation model. (B-E) Perfused human and murine vessels were identified by tail-vein injection of a mixture of rhodamine (red)–conjugated UEA-I and FITC (green)–conjugated GS-IB4. (B) Representative confocal images of 3 distinct patterns of lectin-labeled vessels in the implants at pretransplantation day 7. White arrowheads indicate human, murine, and chimeric vessels. (C) Representative confocal images of lectin-labeled vessels in the implants at multiple time points. White dotted line in the day 3 panel of pretransplantation indicates the border of implant. (D) Graph of MVD before and after transplantation (n = 3-8; mean ± SEM). *Significant difference (P ≤ .05) between groups for total MVD. †Significant difference (P ≤ .05) between groups for human MVD. (E) Representative confocal images of GFP/UEA-I (rhodamine)–positive vessels in the implants at day 7 after transplantation into GFP-transgenic mice. White dotted line indicates the border of implant. Scale bars represent 100 μm.
