Analysis of the expression of AI467606 in mouse embryos and adult hematopoietic organs. (A) FACS analysis of CD41 and AI467606-GFP coexpression in single embryos at E7.5, E8.5, yolk sac at E9.5 and E10.5. (B) GFP and control antibody immunohistochemistry of paraffin sections of transgenic embryos at E12.5. (C) FACS analysis of AI467606-GFP coexpression with CD41, CD45, CD34, Ter119, cKit, Gr-1, and Mac-1 in E12.5 fetal liver. Numbers indicate percentages of respective populations. (D) GFP FACS profile of E8.5 embryos and FACS sorting gates. Number of colonies generated by each cell population in methylcellulose. Mean numbers of colonies from 3 dishes of a representative experiment are shown. Error bars represent SEM (n = 3), Pre corresponds to the total population (Pre-sort). (E) GFP and control antibody immunohistochemistry of paraffin sections of bone marrow, spleen, and thymus of transgenic embryos. (F) FACS analysis of AI467606-GFP coexpression with B220 B-cell marker, Mac-1 myeloid marker, Ter119 erythroid marker, and CD4 and CD8 T-cell markers in bone marrow (left), spleen (middle), and thymus (right) of AI467606-GFP mice. Numbers indicate percentages of respective populations.