αβNKT cells express TCRγ mRNA. (A-B) Expression of TCRVγ genes normalized to β-actin in the indicated thymocyte subpopulations, measured by real-time RT-PCR in arbitrary units relative to NKT Vγ4 (AU). (C) Numbers in dot plots show percentages and indicate the purity of thymocyte subsets, sorted as indicated in “Cell preparation, flow cytometry, and cell sorting,” from which mRNA was isolated. Sorted NKT cells were not contaminated with γδT cells. Proportions of given subpopulations in unsorted thymocytes are also shown. Data are representative of at least 2 independent experiments.