Figure 5
Dysregulation of CDKN1a (p21) and Arf in Dmtf1−/− KSL cells. (A) Sorted KSL cells from null or WT mice were cultured with SCF/TPO/Flt3-ligand/IL-3. Cells were harvested at the indicated time points and the expression of cdkn1a (p21) and Arf were evaluated by quantitative RT-PCR. (B) One thousand sorted KSL cells from KO or WT mice were cultured for 72 hours under the same conditions described in panel A, and then transplanted into lethally irradiated mice in competition with 3 × 105 B/J MNCs. Chimerism was analyzed by flow cytometric analysis (n = 5 in each group).
