Figure 2
Cell-cycle alterations in steady-state BM of Dmtf1−/− mice. (A) Cell-cycle analysis in steady-state BM was performed with DAPI. Percentages of cycling cells (S/G2/M) are shown for each fraction (n = 4). *P < .05. (B) Cell-cycle status of KSL cells was evaluated with Hst/Py staining. WT or Dmtf1−/− BM low-density cells were stained for surface antigens followed by Hst/Py staining. Dot plots in panel B show representative results of Hst/Py staining of WT and KO cells depicting G0 (bottom left quadrant), G1 (top left quadrant), and S/G2/M (top right quadrant) in KSL cells. Bar graph to the right shows the percentage of cells in the 3 phases of cell-cycle as average ± SD. *P < .03. (C) Lin− cells from WT and Dmtf1−/− mice were cultured without cytokines in serum-supplemented medium for the indicated times and stained with annexin V/propidium iodide (PI). Data shown are the mean percentage ± SD of annexin V+/PI− and annexin V+/PI+ cells. No significant differences were observed between WT and Dmtf1−/− mice (n = 3).

Cell-cycle alterations in steady-state BM of Dmtf1−/− mice. (A) Cell-cycle analysis in steady-state BM was performed with DAPI. Percentages of cycling cells (S/G2/M) are shown for each fraction (n = 4). *P < .05. (B) Cell-cycle status of KSL cells was evaluated with Hst/Py staining. WT or Dmtf1−/− BM low-density cells were stained for surface antigens followed by Hst/Py staining. Dot plots in panel B show representative results of Hst/Py staining of WT and KO cells depicting G0 (bottom left quadrant), G1 (top left quadrant), and S/G2/M (top right quadrant) in KSL cells. Bar graph to the right shows the percentage of cells in the 3 phases of cell-cycle as average ± SD. *P < .03. (C) Lin cells from WT and Dmtf1−/− mice were cultured without cytokines in serum-supplemented medium for the indicated times and stained with annexin V/propidium iodide (PI). Data shown are the mean percentage ± SD of annexin V+/PI and annexin V+/PI+ cells. No significant differences were observed between WT and Dmtf1−/− mice (n = 3).

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