Modulation of AKT phosphorylation by thrombospondin 1 in LBW-ECFCs. pAKT and total AKT protein were determined by immunoblot as described in “Methods.” (A) Representative experiment of pAKT and total AKT protein expression in 2 independent < 1500-g LBW-ECFCs and > 2700-g CT-ECFCs. A total of 40 μg of cell lysate was loaded on a 12% acrylamide gel. After transfer, membranes were probed with anti-pAKT, stripped and blotted with antitotal AKT, stripped again, and probed with β-tubulin for charge control. (B) Representative immunoblots of pAKT and total AKT performed on cell lysates (40 μg) from LBW-ECFCs transfected with scr or THBS1 siRNAs. (Inset) Quantification of band intensity of 3 independent immunoblots. Data are mean ± SEM. *P < .05. **P < .01.