Effect of H60 vaccination on T_M recipients. B6.H60 → actH60 mice were irradiated and reconstituted with T cell–depleted C3H.SW BM with no T cells or 5 × 10⁵ CD8⁺ T_M from DEC-H60–vaccinated or unmanipulated C3H.SW donors. Additional positive GVHD control groups received 5 × 10⁵ or 10⁶ T_N from unmanipulated C3H.SW donors. As measured by weight change (A) and survival (B), neither CD8⁺ T_M from DEC-H60–vaccinated nor those from unmanipulated C3H.SW mice induced clinical GVHD. P > .07 comparing percent weight change of T_MH60 and BM controls at all days except days +3 and +24. P ≥ .29 comparing weight change in T_MH60 and T_M recipients at all days except day +3. P ≥ .4561 comparing survival in BM alone with the T_MH60 or T_M group; P ≤ .0109 comparing BM alone recipients with either T_N group. Neither T_M nor T_MH60 cells induced histopathologic skin, ear, or colon GVHD (P > .12 compared with BM alone controls). However, T_MH60 induced GVHD in the liver compared with T_M or BM alone groups (P = .0015). T_M and T_MH60 recipients had similar scores in all other tissues (P ≥ .2). In contrast, both T_N doses induced significant ear GVHD and 10⁶ T_N induced skin, colon, and liver pathology (P ≤ .04 compared with BM alone controls). Tet^H60+ cells expanded in T_MH60 recipients. (D) Representative flow cytometry at day +14. Data are combined from 2 experiments with similar results.