Abnormal presence of FLNa in platelets of 3 patients. Two-color confocal microscopy comparing the distribution of antibodies detecting FLNa (green fluorescence) and VWF (red fluorescence) in fixed and permeabilized platelets. Note that for P1 and P2, the platelets lacking FLNa are the largest, whereas for P3 there is a loss of the more peripheral FLNa organization typical for control platelets. (Right and bottom) Projections of the images on the z-axis showing a cottony appearance. Western blotting with anti-FLNa antibody. Note the signs of FLNa degradation that were more extensive for P3.