SD1 cells produce CNS disease in a mouse model. (A) Representative images of 3 mice from a cohort of 10 animals/cell line are shown. SD1, REH, and SupB15 cause disease in SCID-Beige mice, with SD1 producing the highest disease burden as measured by bioluminescence. The lower panel shows the representative luminescence activity in brain (b) and skull (s) for each cell line. Bioluminescence values are indicated as photons/cm2/s. (B) Histological sections of the brain of a representative SD1-engrafted SCID-Beige mouse. Sections were stained with anti-GFP antibody and show infiltration in the nasal region and cranial marrow cavity. Cells can be seen within blood vessels (bv) of the brain parenchyma and within the choroid layer of the eye. (C) Section through the brain of a representative mouse transplanted with SD1 cells showing cells within the lateral ventricles (top and middle panels) and infiltrating along the optic nerve (bottom panel). Images were captured on a Mirax scanner (3Dhistech) using a Zeiss Fluor 20×/0.75 NA objective lens and a Marlin (Allied Vision Technologies) color camera. The system uses the 3DHistech scan software, and images were viewed and exported using 3DHistech panoramic viewer software.
